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Chromatin: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 189

Edited By: Hannah Gould

Oxford University Press

Paperback | Apr 1998 | #83991 | ISBN: 0199635986
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $92/€80 approx
Hardback | Dec 1998 | #83990 | ISBN: 0199635994
Out of Print Details

About this book

Chromatin comprises the complex of nucleic acids and proteins that make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin should find this book useful. It should also be of interest to experienced researchers.

"The surge of research in this topic is reflected in the recognition that chromatin is the target of regulatory enzymes that can switch states of activity by reversibly stabilizing or disrupting inherent structural features. These advances lead to a compelling need for straightforward methods capable of assembling naked DNA into chromatin in vitro, and of achieving the necessary quality control to determine the physical structure and composition of the chromatin that is assembled. The research goal for many investigators is to use chromatin templates to reconstitute more accurately the natural regulatory environment for the study of events in the nucleus. Therefore, it is also essential to have methods capable of determining the organization of genes within chromatin in vivo. This book goes a long way toward meeting these needs. . . . I hope that both new and established investigators will make use of this volume."--The Quarterly Review of Biology


1. Isolation and fractionation of chromatin and linker histones; 2. Extraction and separation of core histones and non-histone chromosomal proteins; 3. Mapping chromatin structure in Drosophila; 4. In vivo footprint and chromatin analysis by LMPCR; 5. Analysis of nuclear scaffold attachment regions; 6. Electron microscopy of chromatin; 7. Counting nucleosome cores on circular DNA using topoisomerase I; 8. In vitro reconstitution of chromatin and analysis of nucleosome positioning; 9. Transcriptional and structural analysis of chromatin assembled in vivo; 10. An assembly of chromatin and nuclear structures in Xenopus egg extracts; Appendix: List of Suppliers; Index

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