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About this book
This work describes chromotographic and electrophoretic principles and procedures for analyses of various amphiphilic and hydrophilic biomolecules, particulary for food analysis. Providing basic information, including general sample preparation, the book then goes on to describe individual analytical methods and exemplify the strategy and methodologies employed for the analyses. The theory necessary to understand the methods and interpretation of results is also included, as are instructions on experiments
Contents
General aspects of experimental biochemistry; buffers and micelles; binding, association, dissocation, and kinetics; extraction of native low- and high-Mr biomolecules; spectroscopy and detection methods; liquid chromatography; ion-exchange chromatography; electrophoresis; high-performance capillary electrophoresis; analytical determination of low-Mr compounds; protein purification and analysis; immunichemical techniques; analysis of dietary fibre.
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