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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Complement: A Practical Approach

Handbook / Manual
Edited By: Alister W Dodds and Robert B Sim
Publisher: IRL Press
Complement: A Practical Approach
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  • Complement: A Practical Approach ISBN: 9780199635399 Paperback Jul 1997 Usually dispatched within 2-3 weeks
  • Complement: A Practical Approach ISBN: 9780199635405 Hardback Dec 1997 Out of Print #84006
Selected version: £69.99
About this book Contents Customer reviews Related titles

About this book

The complement system is a collection of plasma enzymes that interact in a complex way as a reaction to cell injury, and is central to the immune response. The enzymes that make up the system and their interactions are very widely studied. This text contains detailed step-by-step protocols for analysis of the complement system, and is aimed at new and experienced workers in complement research, as well as clinicians with an interest in immunological diseases. The areas covered include functional and immunochemical methods for the quantitation of complement components, inhibitors, receptors, inflammatory effects of anaphylatoxin release, methods for the analysis of complement biosynthesis and for the study of the system in non-humans. Of particular interest to clinicians are chapters on complement and immune complexes, C2 Inh deficiency, and the genetic analysis of this highly polymorphic system. This book is intended for post-graduate and post-doctoral researchers, academic staff, and clinicians in the fields of biochemical immunology and immunological disease.


1. The complement system: an introduction; 2. Haemolytic assays for whole complement activity and individual components; 3. Immunochemical methods for the detection of complement components and complement activation; 4. Identification, functional assessment, and quantitation of receptors and membrane regulatory proteins whose ligands are C3 and C4; 5. C1 inhibitor and HANe; 6. Formation and regulation of the membrane attack complex; 7. Purification of complement components; 8. The anaphylatoxins; 9. Complement genetics; 10. Biosynthesis of complement components; 11. Complement and immune complexes; 12. Non-human complement

Customer Reviews

Handbook / Manual
Edited By: Alister W Dodds and Robert B Sim
Publisher: IRL Press
Media reviews

"To those who are not actively engaged in the study of its biology and function, the complement system has remained one of the most formidable and dreaded subjects to deal with. . . . This manual . . . is therefore a very timely compilation of all the important protocols of assays that deal with the complement system. The book's twelve chapters are written by the most respected authorities in the field, and each chapter will serve the interests of researchers and clinicians. In addition, the book contains a useful list of suppliers and their addresses. . . . This compendium of different protocols for the isolation, purification and the study of complement structure and function will be a much needed manual, and an easy-to-read companion for both beginners and seasoned veterans. With such a wealth of information, this book provides an easily accessible reference source for clinicians, immunologists and students."--The Quarterly Review of Biology

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