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Epithelial Cell Culture: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 166

Edited By: Andrew J Shaw

238 pages, 15 b/w photos, 30 line illus

IRL Press

Paperback | Apr 1996 | #51492 | ISBN: 0199635722
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $92/€79 approx
Hardback | Dec 1996 | #51491 | ISBN: 0199635730
Out of Print Details

About this book

This text describes methods of culturing epithelial cells so as to mimic accurately their behaviour in vivo. The resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxicology and cell biology. The culture technique involves growing cells on a porous substrate, so that the cells can receive nutrients by diffusion from the underlying layer, as they would in vivo.


1. Modelling epithelial tissues; 2. Identification and localization of cytokeratins; 3. Assessment of cell polarity; 4. Xenobiotic metabolism in epithelial cells; 5. Culture and characterization of human endothelial cells; 6. Studying transport processes in aborptive epithelia; 7. A model of glandular epithelium for studying secretion; 8. A model of the blood-testes barrier for studying testicular toxicity; 9. Reconstruction of human skin epidermis in vitro; Appendix 1. Addresses of suppliers; Appendix 2. An in vitro model of the blood-brain barrier for studying drug transport to the brain

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