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Free Radicals: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 168

Edited By: Neville A Punchard and Frank J Kelly

IRL Press

Paperback | Aug 1996 | #83943 | ISBN: 0199635595
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $92/€80 approx
Hardback | Dec 1996 | #83942 | ISBN: 0199635609
Out of Print Details

About this book

This volume brings together practical techniques for detecting and measuring free radicals - the reactive, short-lived molecules now known to play an important role in a large number of human diseases, including heart disease and cancer - and for studying their effects. Consequently, their study in biological systems has increased enormously in recent years, and this book may be of interest to a wide cross-section of biochemists and clinicians.

Introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts for which the protocols are relevant...This book will be not just a laboratory manual but should be a bestseller in the student market.--Times Higher Education Supplement


Introduction; 1. Direct and indirect measurements of free radicals by physico-chemical methods; 1.1 In vitro and in vivo detection of free radicals metabolites with electron spin resonance; 1.2 The application of high-field nuclear magnetic resonance spectroscopy to the detection of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs; 2. Direct and indirect measurement of free radicals by other methods; 2.1 Intrinsic (low-level) chemiluminescence; 2.2 Visual assessment of oxidative stress by multifunctional digital microfluorography; 2.3 Salicylic acid and phenylalanine as probes to detect hydroxyl radicals; 3. Measurement of free radical products; 3.1 Lipids (i): peroxides and other products; 3.2 Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal; 3.3 Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipid peroxidation; 3.4 Proteins (i): determination of carbonyl groups in oxidized proteins; 3.5 Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA; 3.6 Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems; 3.7 Nucleic acids: measurement of products of free radical attack on nucleic acids; 4. Measurement of antioxidants; 4.1 Glutathione; 4.2 Glutathione peroxidase: activity and steady-state level of mRBA; 4.3 Superoxide dismutase; 4.4 Application of deuterium labelling and gas chromatography-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids; 4.5 Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants; 4.6 Measurement of antioxidant gene expression; Appendix

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