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Known world-wide as the standard introductory text to this important and exciting area, the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations.
In addition to a number of informative changes to the text throughout Gene Cloning and DNA Analysis, the chapters on DNA sequencing and genome studies have been rewritten to reflect the continuing rapid developments in this area of DNA analysis:
- In depth description of the next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes
- New material on the use of ChiP-seq to locate protein-binding sites
- Extended coverage of the strategies used to assemble genome sequences
- Description of how the Neanderthal genome has been sequenced and what that sequence tells us about interbreeding between Neanderthals and Homo sapiens
Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.
Part I The Basic Principles of Gene Cloning and DNA Analysis 1
1 Why Gene Cloning and DNA Analysis are Important 3
2 Vectors for Gene Cloning: Plasmids and Bacteriophages 13
3 Purification of DNA from Living Cells 25
4 Manipulation of Purified DNA 47
5 Introduction of DNA into Living Cells 75
6 Cloning Vectors for Escherichia coli 93
7 Cloning Vectors for Eukaryotes 111
8 How to Obtain a Clone of a Specific Gene 135
9 The Polymerase Chain Reaction 157
Part II The Applications of Gene Cloning and DNA Analysis in Research 173
10 Sequencing Genes and Genomes 175
11 Studying Gene Expression and Function 201
12 Studying Genomes 225
Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology 245
13 Production of Protein from Cloned Genes 247
14 Gene Cloning and DNA Analysis in Medicine 269
15 Gene Cloning and DNA Analysis in Agriculture 291
16 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 311
Glossary 329
Index 345
