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Gene expression analysis in the RNA world is a practical guide to the principles and techniques that are essential to RNA-based gene expression analysis. Key features include a detailed presentation of the procedures and methods for triplicates, time course, and recovery studies involving the use of multiple microarrays and correlation analysis between microarray results, RT-PCR, and Northern blotting. Each step of the microarray experimental process from bench work of RNA isolation through to computational and bioinformatics analysis is described in detail.
- Gene expression regulation - the concept and practice of a Rnase-Free Lab
- Total RNA isolation through ultracentrifugation versus commercial kits, RNA integrity assessment
- Using total RNA for gene-specific expression studies using RT-PCR and Northern Analysis
- Using total RNA for genome-wide expression studies using microarray analysis
- Critical comments on bioinformatics software and web-based resources
Jade Q. Clement is an associate professor in the Department of Chemistry at Texas Southern University, USA. She has formal training and experience in a broad range of biomedical fields including molecular biology/biochemistry, basic medicine, public health, epidemiology, microbiology and immunology. Her Ph.D. was in Biomedical Sciences, earned from the University of Texas Graduate School of Biomedical Sciences in conjunction with M.D. Anderson Cancer Center. Dr Clement's recent research focuses on the application of genomic technology in studying cellular and molecular responses in altered environments such as reduced gravity, radiation and toxic material exposures.
Dr Clement has extensive experience in studying nuclear pre-mRNA introns and their metabolic stability in vivo and in vitro as well as transcription regulation by a nucleotide analog DRB in mammalian cells. In addition, Dr. Clement has worked on infectious agents such as Vibrio cholera in aspects of microbiology, immunology, and molecular genetics. Dr Clement's current research interests also include small regulatory RNAs (such as microRNA and siRNA).