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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

High Resolution Chromatography

Handbook / Manual
Edited By: Paul Millner
320 pages, 5 b/w illus, 70 figs
High Resolution Chromatography
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  • High Resolution Chromatography ISBN: 9780199636488 Paperback Apr 1999 Usually dispatched within 5 days
  • High Resolution Chromatography ISBN: 9780199636495 Hardback Apr 1999 Usually dispatched within 2-3 weeks
Selected version: £69.99
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About this book

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.


Separation by charge, size and hydrophobicity; Practice and benefits of microcolumn HPLC; Detection devices; Capillary electrophoresis of peptides and proteins; Lectins as affinity probes; Nucleotide-and dye-ligand chromatography; Synthetic peptides as affinity ligands; Drugs and inhibitors as affinity ligands; Immunoaffinity chromatography; Strategies and methods for purification of Ca2+ -binding proteins; Purification of DNA-binding proteins

Customer Reviews

Handbook / Manual
Edited By: Paul Millner
320 pages, 5 b/w illus, 70 figs
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