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About this book
About this book
Presents the basic concepts and applied techniques of plant cell and tissue culture.
Contents Foreword Preface Chapter 1. Introduction Types of In Vitro Culture Applications of Plant Tissue Culture Chapter 2. Morphogenesis/Organogenesis Introduction Plant Growth Cellular Differentiation Morphogenesis Chapter 3. Micropropagation Definition Stages in Micropropagation Commercial Micropropagation Applications of Micropropagation Chapter 4. Haploid Plant Production In Vitro Anatomy of Anther Anther Culture Androgenesis Chapter 5. In Vitro Pollination and Fertilization Development of Female Gametophyte Pollination Fertilization Embryo Culture Chapter 6. Somatic Hybridization Using Protoplast Technology Introduction Uses of Protoplast Technology Obtaining Protoplasts The Culture of Protoplasts The Cytoplasmic Genomes Common Potential of Protoplast Fusion Chapter 7. Cell Culture and Selection of Desirable Traits Selection of Naturally Occurring Variants in Culture General Selection Strategies Chapter 8. In Vitro Mutagenesis Types of Mutagens Determining the Type and Suitable Concentration of Mutagens The Choice of Plant Tissues for In Vitro Mutagensis Chapter 9. The Origin, Nature, and Significance of Variation in Tissue Culture Introduction The Basis of Somaclonal Variations Causes of Somaclonal Variations Use of Somaclonal Variation in Breeding Prevention of Somaclonal Variation Chapter 10. Cryopreservation and Plant Breeding Introduction Theory and Technology Cryopreservation Protocols for Cold-Hardy and Non-Cold-Hardy Species Storage and Thawing Equipment for Cryopreservation Practical Issues and Strategies Toward Improved Cryoprotection Chapter 11. In Vitro Micrografting Definition of Micrografting Analysis of Compatibility and Incompatibility Phenomena Chapter 12. In Vitro Flowering: Its Relevance to Plant Breeding Factors Influencing In Vitro Flowering Plant Growth Regulators Mineral Nutrients and Other Medium Components Explant, Light, and Other Variables Application of In Vitro Flowering to Plant Breeding Chapter 13. In Vitro Tuberization Introduction Factors Controlling Microtuber Production Practical Aspects of In Vitro Tuberization Chapter 14. Molecular Plant Breeding Types of Molecular Markers Major Objectives of Molecular Breeding Applications of Molecular Markers in Plant Breeding Case Study: Application of Molecular Markers in Barley (Hordeum vulgare) Breeding References Index