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Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment.
Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical chemistry.
- The Sampling and Sample Preparation Problem in Microbial Metabolomics
- Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics
- Uncertainty of Measurement in Quantitative Metabolomics
- GC and Comprehensive Two-Dimensional GC Hyphenated with Mass Spectrometry for Targeted and Non-Targeted Metabolomics
- LC-MS Based Non-Targeted Metabolomics
- The Potential of Ultrahigh Resolution MS (FTICR-MS) in Metabolomics
- The Art and Practice of Lipidomics
- The Role of CE-MS in Metabolomics
- Metabolomics in Practice: NMR-based metabolomics analysis
- Potential of Microfluidics and Single Cell Analysis in Metabolomics (Micrometabolomics)
- Data Processing in Metabolomics
- Metabolic Flux Analysis
- Metabolomics: Application in plant sciences
- Metabolomics and its Role in the Study of Mammalian Systems
- Metabolomics in Biotechnology (Microbial Metabolomics)
- Nutritional Metabolomics
Michael Lämmerhofer is Associate Professor at the Department of Analytical Chemistry of the University of Vienna (Austria). Having obtained his academic degrees from the University of Graz, he spent two years as a post-doctoral fellow at the Department of Chemistry of the University of California, Berkeley (USA). His current research is directed towards the targeted quantitative analysis of extracellular and intracellular metabolites, mainly with focus on applications in industrial biotechnology.
Wolfram Weckwerth is Full Professor and Head of the Department of Molecular Systems Biology at the University of Vienna (Austria). Having obtained his academic degrees from the Technical University of Berlin (Germany), he moved to the Max-Planck-Institute of Molecular Plant Physiology, where he pioneered metabolomic methods and integrated proteomic and computer-based approaches into a systems biology framework. After his habilitation in Systems Biology at the University of Potsdam, he took over a position as a head of a research lab in the German FORSYS systems biology initiative, before founding the Department of Molecular Systems Biology at the University of Vienna in 2008.