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Methods in Arabidopsis Research

Handbook / Manual

Edited By: C Koncz, Nam-Hai Chua and J Schell

About this book

The small weed Arabidopsis is now the object of rapidly growing scientific attention, because of its small genome and well-documented genetic studies, and this book offers a wealth of methodical and environmental information for its study.

... a very useful book ... excellent introductions to the issues they deal with as well as fully detailed descriptions of each respective method with a lot of valuable methodological pointers. " This book contains for everybody who is working with this plant a lot of excellent well-organized information, capped by outstanding index of 40 pages. But also a reader who is not working with Arabidopsis will learn a lot about an interesting plant and modern methods of genetics, as well as some about the recent history of science in a restricted field. A cheap paperback edition as well as the more expensive hardcover edition make this highly recommendable book available for students, too." Johannes Siemens Plant Breeding "Judging by the number of times that my colleagues and I have already referred to this book since it arrived, I can safely say that it will serve as an excellent reference for those researchers already familiar with the field. It should also provide a good introduction for newcomers. I would recommend its purchase." C Dean Journal of Experimental Botany, 1993 "... extremely useful to any researcher involved in cloning genes from Arabidopsis ... This makes this book a useful reference to have in any plant molecular biology laboratory." Vicky Buchanan-Wollaston Plant Growth Regulation, 1993 "I believe the volume will be valuable as well to those studying other plant species. In it we have a book that belongs on the desk of every molecular plant scientist." Peter M Gresshoff Science, 1993 "... the book reviewed here, which represents the first successful attempt in recent years to summarize the molecular genetics of Arabidopsis. This book provides detailed information on research methods used for genetic and molecular analysis in Arabidopsis. It is therefore a valuable reference for individuals interested in using Arabidopsis in their own research programs. David W Meinke Plant Genetics Newsletter, 1995

A Heuristic Glance at the Past of Arabidopsis Genetics (G P Redei); Classical Mutagenesis (G P Redei & C Koncz); Genetic Analysis (M Koornneef & P Stam); Introduction to the Arabidopsis Genome (E M Meyerowitz); Chromosome Walking in Arabidopsis Thaliana Using Yeast Artificial Chromosomes (S I Gibson & C Somerville); Genetic and Physical Linkage of the Arabidopsis Genome: Methods for Anchoring Yeast Artificial Chromosomes. (E Matallana, C J Bell, P J Dunn, M Lu & J R Ecker); Genetic Linkage of the Arabidopsis Genome: Methods for Mapping with Recombinant Inbreds and Random Amplified Polymorphic DNAs (RAPDs) (R S Reiter, R M Young & P A Scolnik); Genome Mapping in Arabidopsis (B M Hauge & H M Goodman); T-DNA Transformation and Insertion Mutagenesis (C Koncz, J Schell & G P Redei); T-DNA Insertion Mutagenesis in Arabidopsis: Seed Infection/Transformation (K A Feldmann); Transposon Tagging in Arabidopsis (G Coupland); Protoplast Transformation and Methods to Create Specific Mutants in Arabidopsis Thaliana (T Altmann, B Damm, U Halfter, L Willmitzer & P-C Morris); Cloning Arabidopsis Genes by Genomic Subtraction (T-P Sun, D Straus & F M Ausubel,); Gene Isolation with the Polymerase Chain Reaction (A Gasch, T Aoyama, R Foster & N-H Chua); Microsequence Analysis of Arabidopsis Proteins Separated by Two-Dimensional Polyacrylamide Gel Electrophoresis: A Direct Linkage of Proteins and Genes (G Bauw, M Van Montagu & D Inze); Analysis of Protein/DNA Interactions (R Foster, A Gasch, S Kay & N-H Chua ); Genetic Definition of Loci Involved in Arabidopsis-Pathogen Interactions (J L Dangl, E B Holub, T Debener, H Lehnackers, C Ritter & I R Crute); Arabidopsis Mutant Collection (M Anderson & B Mulligan).