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Molecular Plant Biology is an all-new replacement for the original Practical Approach book Plant Molecular Biology that was first published in 1988. The rapid advances made in plant sciences during the past decade are reflected by the need to produce a two-volume book to cover all the relevant methodologies. The new book incorporates many of the fundamental procedures outlined in the original book, but these are fully updated to reflect advances in technology and the development of new methodologies. The new books also incorporate many approaches that were not available in the earlier volume. Each chapter has been written by an international expert with current practical expertise in the topics covered. Each book is divided into three sections. The approaches outlined in Volume One cover a wide range of techniques for gene isolation, gene identification and subsequent gene isolation as well as for studies of gene organization. . The methods described range from classical mutagenesis through plant transformation, T-DNA and transposon tagging strategies, genomic subtraction, gene mapping, construction and screening of YAC, BAC and cosmid libraries chromosome in situ, and isolation of cDNA sequences by western and southwestern library screens, and complementation cloning.
PART 1: GENE IDENTIFICATION; 1. Classical mutagenesis in higher plants; 2. Plant transfomrmation; 3. T-DNA tagging; 4. Transposion tagging methods in heterologous plants; 5. Cloning plant genes by genomic subtraction; PART 2: GENE ORGANIZATION; 6. Approaches to gene mapping; 7. Arabidopsis YAC, BAC and cosmid libraries; 8. In situ hybridization to plant chromosomes and DNA fibres; PART 3: LIBRARY SCREENING AND CDNA ISOLATION; 9. PCR cloning approaches; 10. Western and southwestern library screens; 11. Complementation Cloning
This Practical Approach book will have a broad distribution and is essential on each plant molecular biology bench. Journal of Physiology