Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.
the protocols are concise and easy to follow. They are, in fact, extraordinary in their detail Nitric Oxide Protocols is a useful volume, and one that will certainly find its way into many laboratories entering the exciting field of nitric oxide research. - The Quarterly Review of Biology
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