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Nucleic Acid Protocols Handbook

Handbook / Manual

Series: Methods in Molecular Biology

By: Ralph Rapley and John M Walker

Humana Press

Paperback | Dec 1999 | #104089 | ISBN: 0896034593
Availability: Usually dispatched within 1 week Details
NHBS Price: £149.00 $196/€167 approx
Hardback | Dec 1999 | #104090 | ISBN: 0896038017
Availability: Usually dispatched within 1 week Details
NHBS Price: £138.00 $182/€154 approx

About this book

Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors - leading scientists, clinicians, and public health physicians - describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. Timely and comprehensive, "Meningococcal Vaccines: Methods and Protocols" provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus, and to develop, evaluate, and implement successful new meningococcal vaccines.

... the techniques are presented in minute details and in a cookbook-standardized manner ... The personal notes inserted by each contributor provide assurance that the techniques would work in any laboratory... Having all of those protocols updated and tested in practice and bound in one volume will be attractive to many scientists who deal with molecular biology techniques. - Modern Pathology There were over 190 authors involved in writing the protocols, each protocol is presented in outline format an follows a common numbering scheme, making it easy to find relevant sections. Many of the protocols include tables of data or figures, including drawings or photographs of gels One of the most useful sections in each protocol lists notes, which elucidate possible pitfalls, provide troubleshooting tips, or give alternate methods or explanations for steps. This very practical information makes successful duplication of the procedure much more likely than when attempting to follow the often less detailed or less flexible descriptions given in journal articles. this handbook would be extremely useful as a reference in most university libraries. - E-Streams it covers practically all of the very important and large field of nucleic acid research. The volume summarizes, in 10 parts, recent advances in the isolation , analysis and manipulation of nucleic acids. The chapters were contributed by authors from leading laboratories in the field all over the world. The book provides the reader with a large set of ready-to use protocols needed to undertake complex molecular biology experimental project on a large spectrum of biological models involving isolation and analysis of nucleic acids. It is thus an almost indispensable handbook for anybody working in the field of molecular biology and genetics. - Folia Microbiologica extremely useful as a reference in most university libraries. - E-Streams


Part I Nucleic Acids Extraction; Part II Basic Separation and Analysis of DNA; Part III Probe Design, Synthesis and Labeling; Part IV RNA Analysis Techniques; Part V Gene Library Construction and Screening; Part VI DNA Sequencing; Part VII Basic Polymerase Chain Reaction Methods; Part VIII Analyzing Genes, Mutations and Protein Interactions; Part IX Mutagenesis, Transcription and Translation In Vitro; Part X Gene Localization and Mapping In Situ;

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