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This expanded edition includes identification of microalgae used in aquaculture manual. The manual now includes use of microscope, slide preparation, measuring cells, stains, key to genera, description of cells variations, contaminants in cultures and photos. It provides updated step by step instructions for culturing phytoplankton (microalgae) and zooplankton including rotifers, adult brine shrimp, ciliates, copepods, daphnia, Oyster and clams veligers, amphipods, mysid shrimp and microworms. Technical data and illustrations support the instructions in a clear understandable format. Sections include trouble shooting and reasons for failure. Includes simple culture methods and more intensive, large scale methods and designs.