Plant Epigenetics Methods and Protocols

About this book

The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In this volume, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants.

Contents

Table of Contents
Preface
Contributors
Analysis of DNA Methylation in Plants by Bisulfite Sequencing Analysis of bisulfite sequencing data from plant DNA with CyMATE Analysis of locus-specific changes in methylation patterns using a COBRA (combined bisulfite restriction analysis) assay
Detection of changes in global genome methylation using the cytosine-extension assay
In situ analysis of DNA methylation in plants
Analysis of mutation/rearrangement frequencies and methylation patterns at a given DNA locus using RFLP (restriction fragment length polymorphism)
Isoschizomers and Amplified Fragment Length Polymorphism (AFLP) for the Detection of Specific Cytosine Methylation Changes Analysis of small RNA populations using hybridization to DNA tiling arrays Northern blotting techniques for small RNAs
qRT-PCR of small RNAs
Cloning new small RNA sequences
Genome-wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A -- ChIP-chip Molecular Methods
Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitation and DNA microarray hybridization (ChIP-chip). Part B -- ChIP-chip data analysis
Metaanalysis of ChIP-chip data
Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
cDNA Libraries for Virus-Induced Gene Silencing
Detection and quantification of DNA strand breaks using the ROPS (random oligonucleotide primed synthesis) assay
Reporter gene-based recombination lines for studies of genome stability
Plant transgenesis

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