Completely updated and substantially rewritten edition of this manual containing fundamental and recently-described techniques in the area of plant molecular biology.
In vitro methods of gene transfer to plant cells; R. Bilang, A. Kloti, M. Schrott, I. Potrykus, P. Christou, G. Neuhaus. Agrobacterium-mediated gene transfer to plant cells; J. Velten, C. Koncz, N. Martini, L. Szabados, M. Hrouda, A. Bachmair, J. Schell, C. Koncz, P. Hooykaas, S.B. Gelvin, Chang-Nong Liu, L.A. Castle, R.O. Morris, M. Hinchee. Viral mediated gene transfer; C.M. Kearney, S.N. Chapman, W.O. Dawson, D.M. Bisaro, B. Gronenborn. Selectable and screenable markers for plant transformation; M. van Montagu, T.Teeri, Y. Dessaux, A. Petit. Nucleic acid extraction from plant tissue; S.O. Rogers, A.J. Bendich, G.E. Hall Jr., S. Spiker, S.A. Mackenzie, C.A. Price, T. Bisseling. Transcription and translation systems; I.E. Somssich, K. Yamazaki, J.M. Vlak. Blotting and gene detection systems; J. Memelink, K.M.M. Swords, L.A. Staehelin, J.H.C. Hoge, W. Werr, S.J. Karcher, J. Slightom, R.F. Drong, P.P. Chee. In situ hybridization and immunodetection; N.B. Duck, K. van den Bosch, S. Hinnisdaels, M. Jacobs, A. Mouras. Cloning and detection of DNA sequences from large DNA molecules; M.A. Snaed, P.L. Kretz, J.M. Short, G.B. Martin, P. Zabel. Protein-nucleic acid interaction analyses; K. Mikami, H. Takase, M. Iwabuchi, S.J. Martino-Catt, S.A. Kay, R.J. Ferl, A.-L. Paul, E. Lam, L. Meisel. Subcellular targeting of proteins; B.D. Bruce, S. Perry, J. Froehlich, K. Keegstra, M.A. Boutry, J.D. Dombrowski, L. Gomez, M.J. Chrispeels, N.V. Raikhel, V. Citovsky. Gene tagging using transposons; W.-E. Lonnig, P. Huijser, J. Hille, K. Feldman.
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