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Immunocytochemistry and Related Techniques presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes.
Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research.
1. The Evolution of Immunocytochemistry in the Dissection of Neural Complexity Adalberto Merighi and Laura Lossi
Part I
2. Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System Mareike Selcho and Christian Wegener
3. Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris Giovanna Ponte and Graziano Fiorito
4. Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain Nobuhiko Miyasaka, Noriko Wakisaka, and Yoshihiro Yoshihara
Part II
5. Immunocytochemical Phenotype of Differentiating Neurons Andrea Diana and Antonio Carai
6. Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus Oliver von Bohlen und Halbach
7. Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogues. Benefits and Limitations Alvaro Duque and Pasko Rakic
8. Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System Armin Schneider
Part III
9. In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis and Autophagy Maria Grazia Bottone, Francesco Paolo Fanizzi, and Graziella Bernocchi
10. Immunocytochemical Detection of Intraneuronal Ass Peptides in Mouse Models of Alzheimer's Disease Oliver Wirths and Anika Saul
11. Purification and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS) Chrystian Junqueira Alves, Alexandre Baldasserini Guimaraes, Jessica Ruivo Maximino, Sergio Paulo Bydlowski, and Gerson Chadi
12. Immunocytochemistry of Microglial Cells Dmitrii E. Korzhevskii, Olga Kirik, and Elena Sukhorukova
13. Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage Jean-Pierre Louboutin
Part IV
14. Quantification of Immunocytochemical Colocalization in Neurons Brad R. Rocco and Kenneth N. Fish
15. Immunogold Electron Microscopic Quantification of Small Molecular Compounds and Proteins at Synapses and Other Neural Profiles Max Larsson, Linda Hildegard Bergersen, and Vidar Gundersen
16. Combined Immunocytochemistry and Tracing of Neural Connections Taisuke Miyazaki and Masahiko Watanabe
17. Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing and Electrophysiology Alberto Granato and Andrea De Giorgio
18. Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices Silvia Alasia, Adalberto Merighi, and Laura Lossi
19. Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse Brain Ruth M. Empson, Malinda L. S. Tantirigama, Manfred J. Oswald, Stephanie M. Hughes, and Thomas Knopfel
Part V
20. Array Tomography: A Novel High-Resolution Immunofluorescence Technique Mariano Soiza-Reilly
21. Single Molecule Localization Microscopy to Study Neuronal Microtubule Organization Bas M. C. Cloin, Casper C. Hoogenraad, Marina Mikhaylova, and Lukas C. Kapitein
22. Studying Membrane Dynamics of Proteins Using Quantum Dots Martin Heubl, and Sabine Levi
23. Optical Protein Patterning Santiago Costantino
