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Protein Function: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 175

Edited By: TE Creighton

356 pages, Figs, tabs

IRL Press

Paperback | Dec 1997 | Edition: 2 | #84014 | ISBN: 019963615X
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $93/€78 approx
Hardback | Dec 1997 | Edition: 2 | #67460 | ISBN: 0199636168
Out of Print Details

About this book

The companion to this book, "Protein structure: a practical approach", shows methods of determining the structure of a newly discovered protein, but that is only part of the story. This text completes the picture by providing the techniques for elucidating the functional properties of proteins, which can vary enormously, from simple structural roles to catalysis, from information transmission to energy control. This collection of tried-and-tested protocols will be applicable to many proteins, and can be performed in an average laboratory, without the need for specialized equipment.

"This edition . . . has been updated from the original 1989 edition . . . [N]ew chapters have . . . been added to reflect recent improvements in the techniques of molecular biology. . . . The ten chapters roughly focus on five areas. One of the main topics is the chemical modification of proteins . . . Another section considers techniques involved in the detection and quantification of ligand-receptor binding interactions. A third focuses on the purification and analysis of sequence-specific DNA binding proteins. . . . One of the new additions is a discussion of the techniques involved in the overexpression of cloned genes in E. coli and of those used in protein refolding. These chapters are particularly useful, as they contain warnings of numerous pitfalls as well as valuable suggestions that would aid researchers new to these areas. A final addition is a chapter on the identification of proteins by using two-dimensional electrophoresis."--The Quarterly Review of Biology


1. Identifying proteins by two-dimensional gel; electrophoresis; 2. Expressing cloned genes in Escherichia coli; 3. Folding proteins; 4. Ligand binding; 5. Ligand blotting; 6. Affinity labelling; 7. Chemical cross-linking and protein function; 8. Analysis of sequence-specific DNA-binding proteins; 9. Purification of sequence-specific DNA-binding proteins by affinity chromatography; 10. Chemical modification

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