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As more genetic and biochemical information about the protein components of cells accumulates, the analysis of protein-protein interactions is becoming increasingly important. This manual presents a wide range of techniques for identifying and analyzing these interactions, starting with standard molecular and biochemical techniques, and progressing to biophysical and computational approaches and therapeutic and other post-genomic applications. This manual is designed to complement the information in the Molecular Cloning manual, and is presented in the same clear, user-friendly format. It should be a useful resource for investigators studying interacting sets of proteins and their physiologic significance in a wide range of experimental systems.
Section One: The biological context for protein interaction studies; physiological indicators of protein-protein interaction. Section Two: Standard technologies used to identify and characterize protein-protein interactions. Section Three: Biophysical approaches to identify and characterize protein-protein interactions. Section Four: Recent developments and other tools - reviews and short protocols. Section Five: Protein-protein interactions - computation and the future.