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RNA-Protein Interactions: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 192

Edited By: Christopher WJ Smith

341 pages, Illus, figs

IRL Press

Paperback | Jul 1998 | #82086 | ISBN: 0199636508
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £69.99 $92/€78 approx
Hardback | Dec 1998 | #83936 | ISBN: 0199636516
Out of Print Details

About this book

RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field.


1. Applications of chemically synthesized RNA; 2. Preparation of RNA-Protein complexes for X-ray crystallography and NMR; 3. Uses of site-specifically modified RNAs constructed by RNA ligation; 4. The Electrophoretic Mobility Shift Assay for RNA Binding Proteins; 5. Affinity methods for isolating RNA-binding proteins; 6. Synthetic lethal/enhancer screening to identify snRNA-protein and protein-protein interactions in yeast pre-mRNA splicing; 7. Detecting RNA-Protein Interactions and Isolating cDNA Clones by Northwestern Screening; 8. A three-hybrid system to detect and analyse RNA-protein interactions in vivo; 9. In vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay; 10. Foot-printing and modification-interference analysis of binding sites on RNA; 11. Analysis of RNA-Protein and RNA-RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques; 12. In vitro selection of nucleic acid ligands

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