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About this book
About this book
This 1986 book describes the state of research in the area of secondary metabolism in plant cell and tissue culture. Such cultures are a major tool in horticulture and agriculture, and in the chemical industry. The wide range of applications is reflected in the articles presented here, which are grouped according to topic and derive from the meeting held by The International Association of Plant Cell and Tissue Culture in Sheffield in July 1985. This book was a major contribution to the development of plant biotechnology. It will remain of interest to researchers today.
Preface; Acknowledgements; Part I. Secondary Products from Plant Cell Cultures: 1. Aspects of alkaloid production by plant cell cultures; 2. The synthesis and biotransformation of monoterpenes by plant cells in culture; 3. Molymetric adsorbents stimulate anthraquinone production by cell suspension cultures of Cinchona ledgeriana; 4. Biopolymer production by algal cells; 5. Antimicrobial agents from plant cell cultures; Part II. Growth and Differentiation in Relation to Secondary Metabolism: 6. Flavour production in onion tissue culture; 7. Kinetics of growth and alkaloid accumulation in Cartharanthus roseus cell suspension cultures; 8. Differentiation and monoterpene biosynthesis in plant cell cultures; 9. The effect of osmotic stress on growth and alkaloid accumulation in Catharanthus roseus; 10. The relationship between tropane alkaloid production and structural differentiation in plant cell cultures of Atropa belladdona and Hyosyamus muticus; Part III. Biochemistry of Secondary Metabolism: 11. Modulation of the phenyl propanoid pathway in bean (phaseolus vulgaris) cell suspension cultures; 12. Interactions and interelationships between primary and secondary metabolism; 13. Co-ordinated induction of enzymes of secondary metabolism; 14. The biotransformation of monoterpenoids by plant tissue culture; 15. Biosynthesis of phytoalexins and sterols in potato cell suspension cultures; 16. Studies on the regulation of Casaicin biosynthesis in immobilised cell cultures and the developing fruit of the chilli pepper, Capiscum frutescens; 17. The elicitation of phytoalexins in cultures of Capisum annum and Nicotiana tabacum; 18. Studies on alliinase from onion (Allium cepa) and the potential use of onion cell cultures as an enzyme source in onion flavour production; 19. Uptake, binding and metabolism of morphinan alkaloids by cell cultures of Papaver somniferum; Part IV. Mass Cell Culture and Immobilized Plant Cell Systems: 20. The production of secondary metabolites by immobilized plant cells; 21. Immobilization technology; 22. Studies of environmental features of immobilized plant cells; 23. Product release from plant cells grown in culture; 24. Rheological properties of plant cell suspension cultures; 25. Description of a system for the continuous cultivation of plant cells; 26. The economics of mass cell culture; Part V. Selection, Stability and Variation in Secondary Metabolism: 27. Somaclonal variation; 28. Genetic basis of instability in cell cultures; 29. A rapid screening technique for the selection of high yielding capsaicin cell lines of Capiscum frutescens mill.; 30. Selection studies of Catharanthus roseus; 31. Variability and stability of cell cultures of Capiscum frutescens; 32. Selection for resistance to late blight in celery; 33. effects of modification of the primary precursor level by selection and feeding on indole alkaloid accumulation in Catharanthus roseus; 34. Long term stability of alkaloid productivity in cell suspension cultures of Catharanthus roseus.