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In this incisive, concise overview of this booming field, the editors - two of the leading figures in the field with a proven track record - combine their expertise to provide an invaluable reference on the topic. Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies.
Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary. This is equally accessible to both experienced scientists and newcomers to the field.
TRANSCRIPTOME ANALYSIS Single-Cell Expression Profiling AFLP-based RNA Fingerprinting The SAGE family: SAGE, LongSAGE and SuperSAGE DeepCAGE: High-Throughput Gene Expression Analysis for Transcription Start Site and Promoter Identification Paired End diTagging (GIS-PET) for Transcriptome Characterization High-Throughput Functional Screening of Genes in planta Microarrays as Tools to Decipher Transcriptomes in Symbiotic Interactions BeadArray-based Gene Expression Analysis Genome-wide Analysis of mRNA expression by Fluorescent Differential Display Quantitative Real-time RT-PCR GENE SILENCING, MUTATION ANALYSIS, AND FUNCTIONAL GENOMICS RNA Interference for Functional Genomics Extending Functional Genomics: VIGS for Model and Crop Plants Identification of Expressed Genes by Mutation: TILLING Identification of Expressed Genes by Mutation: Transposon Tagging Fast Neutron Mutagenesis COMPUTATIONAL ANALYSIS Bioinformatics Tools to Discover Co-expressed Genes in Plants Promoter Analysis Integration and Phylogenomics Structural, Functional, and Comparative Annotation of Plant Genomes Large-Scale Genomic Sequence Comparisons and Gene Identification with ClustDB EMERGING TECHNOLOGIES Nanotechnologies and Fluorescent Proteins for Functional Genomics Next Generation Sequencing Technologies 454 Sequencing for Functional Genomics
Gunter Kahl is Professor for Plant Molecular Biology in Frankfurt am Main, Germany. He has served in expert missions for IAEA, FAO and UNESCO across the world and is author of more than 250 scientific publications, including several Wiley-VCH books. Khalid Meksem is Professor at the Department of Plant, Soil & General Agriculture of Southern Illinois University. He is associate editor of the Journal of Biomedicine and Biotechnology and chaired the Functional Genomic Workshop at the International Plant & Animal Genome Conference in San Diego. He is reviewer for several granting agencies, including the National Science Foundation and the US Department of Agriculture.
... I recommend this book to any plant researcher who is interested in incorporating functional genomics approaches or who is looking to expand their repertoire. I especially liked that most chapters include detailed 'recipes', which show that anyone with a standard wet laboratory can do most of the procedures... a very useful volume... -The Quarterly Review of Biology, March 2010