This popular lab manual that for years has taught students the basics of working in a lab and how to safely handle microorganisms, has been completely redesigned and updated. All labs have been expanded and reorganized to fit neatly into new sections of the lab, such as "Fungi", "Viruses", or "Biotechnology", and are presented from easiest to most challenging. New introductions for each section provide the appropriate context for the labs and connects the lab concepts to the core text, Fundamentals of Microbiology, Eleventh Edition. The most exciting update are the new videos available with the Lab Manual that teach common lab skills that tie to the labs in the manual. Students will clearly see how to work safely in the lab setting, how to use operating equipment, how to swab cultures, and many more lab skills. New photos and micrograph examples and new exercises and assessments only further enhance the eleventh edition of Laboratory Fundamentals of Microbiology, making it the easy choice for microbiology lab courses.
Part I Laboratory Safety
Lab 1 Safety overview: Biohazards
Lab 2 Best practices in safety
Lab 3 Student safety contract
Part II Laboratory Techniques and Skills
Lab 4 Culture transfer techniques
Lab 5 Pure culture techniques
Lab 6 Pour plate technique
Lab 7 Solution Transfer
Lab 8 Serial Dilutions
Part III Microscopy
Lab 9 Parts of the microscope
Lab 10 Observation of prepared slides
Part IV Bacterial Staining Techniques
Lab 11 Preparation of a bacterial smear
Lab 12 Simple stain technique
Lab 13 The negative stain technique
Lab 14 The Gram stain technique
Lab 15 Bacterial structures – Spore stain
Lab 16 Bacterial structures – Capsule stain
Lab 17 Bacterial structures – Bacterial motility, A. Hanging drop B. Motility test agar technique
Part V Viruses and Eukaryotic Microorganisms
Lab 18 The effect of bacteriophages on bacteria
Lab 19 Plaque formation
Lab 20 Identification of bacteriophages from sewage
Lab 21 Fungi: Molds
Lab 22 Fungi: Yeasts
Lab 23 Protozoa and multicellular parasites
Part VI Control of Microorganisms
Lab 24 The effect of physical agents on bacteria: Heat tolerance
Lab 25 The effect of physical agents on bacteria: Ultraviolet light
Lab 26 The effect of physical agents on bacteria: Drying
Lab 27 The effect of physical agents on bacteria: Other physical agents, A. Incineration B. Osmotic pressure C. Cold temperature D. Autoclaving
Lab 28 The effect of chemical agents on bacteria: Antiseptics and disinfectants
Lab 29 The effect of chemical agents on bacteria: Metals
Lab 30 The effect of chemical agents on bacteria: Other chemical substances
Lab 31 Effect of lysozyme on bacteria
Lab 32 Evaluation of disinfectants and antiseptics: Effectiveness of disinfectants on inanimate objects
Lab 33 Effectiveness of disinfectants on hand washing and the skin surface
Lab 34 Mouthwashes and oral bacteria
Lab 35 The effect of antibiotics on bacteria
Part VII Measuring Population Growth
Lab 36 Estimating the number of bacterial cells in a population, • The Petroff-Hauser Chamber, • The Standard Plate Count
Lab 37 Microbial growth: Analysis of a bacterial growth curve, • Bacterial growth dynamics, • Plotting a growth curve and determining the generation time
Part VIII Medical Microbiology
Lab 38 The genus Mycobacterium: Colony and cellular morphology
Lab 39 The genus Mycobacterium: Acid-fast stain technique
Lab 40 The genus Mycobacterium: Cold acid-fast stain technique
Lab 41 The genus Streptococcus: Streptococci from the upper respiratory tract
Lab 42 The genus Streptococcus: Streptococci from the oral cavity
Lab 43 The genus Neisseria
Lab 44 The genus Staphylococcus: Isolation of Staphylococci
Lab 45 The genus Staphylococcus: Differentiation between Staphylococcal species
Lab 46 The enteric bacteria: Isolation of enteric bacteria
Lab 47 The enteric bacteria: Differentiation of enteric bacteria on TSI agar
Lab 48 The enteric bacteria: The IMViC series, A. Indole test B. Methyl red test C. Voges-Proskauer test D. Citrate test
Lab 49 The enteric bacteria: Rapid identification of enteric bacteria
Lab 50 The genus Bacillus
Lab 51 The genus Clostridium
Lab 52 The genus Lactobacillus: Isolation of Lactobacilli
Lab 53 The genus Lactobacillus: Caries susceptibility test
Part IX Identification of a Bacterial Unknown
Lab 54 Bacterial structural characteristics
Lab 55 Bacterial culture characteristics
Lab 56 Biochemical characteristics of bacteria: Carbohydrate fermentation
Lab 57 Biochemical characteristics of bacteria: Starch digestion
Lab 58 Biochemical characteristics of bacteria: Catalase production
Lab 59 Biochemical characteristics of bacteria: DNA digestion
Lab 60 Biochemical characteristics of bacteria: Hydrogen sulfate production
Lab 61 Biochemical characteristics of bacteria: IMViC series
Lab 62 Biochemical characteristics of bacteria: Urea digestion
Lab 63 Biochemical characteristics of bacteria: Lipid digestion
Lab 64 Biochemical characteristics of bacteria: Casein digestion
Part X Bacterial Genetics
Lab 65 Bacterial Genetics: Mutations and the Ames test – Gradient plate technique
Lab 66 Bacterial Genetics: Mutations and the Ames test – Mutagenic effect of ultraviolet light
Lab 67 Bacterial Genetics: Mutations and the Ames test – DNA repair
Lab 68 Bacterial Genetics: Mutations and the Ames test – The Ames test
Lab 69 Bacterial conjugation
Lab 70 Bacterial transformation
Part XI Immunology
Lab 71 Serology: Agglutination techniques and blood cell identification – Slide agglutination
Lab 72 Serology: Agglutination techniques and blood cell identification – Tube agglutination
Lab 73 Serology: Agglutination techniques and blood cell identification – Hemmagglutination: Determination of blood type
Lab 74 Serology: Agglutination techniques and blood cell identification – Blood smear
Lab 75 ELISA test
Part XII Public Health and Environmental Microbiology
Lab 76 A simulated epidemic: Microbial transmission via fomites
Lab 77 A microbial hunt
Lab 78 Transmission of microorganisms through toilet paper
Lab 79 Disinfection of Drinking Water
Lab 80 Microbiology of foods: Food preservation with salt and garlic
Lab 81 Microbiology of foods: Standard plate count of food products
Lab 82 Microbiology of foods: Fermentation of wine and beer
Lab 83 Microbiology of milk and dairy products: Standard plate count of milk
Lab 84 Microbiology of milk and dairy products: Coliform plate count of milk
Lab 85 Microbiology of milk and dairy products: Methylene blue reduction test
Lab 86 Microbiology of milk and dairy products: Preparation of cheese
Lab 87 Microbiology of milk and dairy products: Preparation of yogurt
Lab 88 Microbiology of milk and dairy products: The natural bacterial content of milk
Lab 89 Microbiology of water: Presumptive test by the MPN method
Lab 90 Microbiology of water: Confirmed test
Lab 91 Microbiology of water: Completed test
Lab 92 Microbiology of water: Membrane filter technique
Lab 93 Microbiology of water: Preparation of a biofilm
Lab 94 Microbiology of soil: Isolation of Rhizobium from legume roots
Lab 95 Microbiology of soil: Ammonification of soil microorganisms
Lab 96 Microbiology of soil: Isolation of Streptomyces from soil
Lab 97 Microbiology of soil: Antibiotic production by Streptomyces
Lab 98 Microbiology of soil: Plate count of soil bacteria
Lab 99 Microbiology of soil: Microbiology ecology in the soil – the Winogradsky column
Jeffrey Pommerville is Professor of Biology and Microbiology at Glendale Community College (GCC) in Glendale, Arizona, where he teaches microbiology and introductory biology. He also has served as the Course Assessment Coordinator for the Biology Department at GCC. Before coming to GCC, he was on the biology faculty at Texas A&M University. Dr. Pommerville received his BS and PhD from the University of California-Santa Barbara. Since coming to GCC, Dr. Pommerville has served as principal investigator on several NSF grants, including Systemic Reform in Science (SyRIS) that developed a strategy to reform and integrate the introductory sciences. He is the author of more than 50 research and education research papers, and was the Perspectives Editor for the Journal of Microbiology and Biology Education, a peer-reviewed journal published by the American Society for Microbiology (ASM). Dr. Pommerville has received numerous professional honors, including: the 2008 Carski Foundation Distinguished Undergraduate Teaching Award from ASM, the Golden Microscope Award from the Leukemia and Lymphoma Society, the Ohaus Award for Innovations in Science Teaching from the National Science Teachers Association, and was one of the first recipients of a Faculty Distinguished Teaching Award from his home institution. He is a member of AAAS, NSTA, NABT, and, of course ASM. Dr. Pommerville is a past co-chair for the ASM’s Conference for Undergraduate Educators as well as a past chair of Division W of ASM.
Dr. Pommerville also is author of Fundamentals of Microbiology (Body Systems Edition), Third Edition (2016), Alcamo’s Microbes and Society, Fourth edition (2016), as well as Encounters in Microbiology, Volume 1 and Volume 2 (2009), and Guide to Infectious Diseases by Body System, Second Edition (2012).