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Transcription Factors: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 647

Edited By: Paul J Higgins

377 pages, 93 b/w & col illus

Humana Press

Hardback | Aug 2010 | #189641 | ISBN-13: 9781607617372
Availability: Usually dispatched within 1 week Details
NHBS Price: £129.99 $171/€145 approx

About this book

In the last few years, significant breakthroughs in transcription research has expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells.

This book describes state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function.


Table of Contents; Preface; Contributors; Part I. Transcription Factor Trafficking and Post-Translational Modifications: Overviews of Mechanisms; 1. A review of post-translational modifications and sub-cellular localization of Ets; transcription factors: possible connection with cancer and; involvement in the hypoxic reponse; Celine Charlot, Helene Dubois-Pot, Tsvetan Serchov, Yves Tourette; And Bohdan Wasylyk; 2. Regulation of transcription factor function by targeted protein degradation: an; overview focusing on p53, c-Myc and c-jun; Jukka Westermarck; 3. Review of molecular mechanisms involved in the activation of the Nrf2-ARE; signaling pathway by chemopreventive agents; Aldo Giudice, Claudio Arra and Maria C. Turco; Part II. Cytoplasmic-nuclear trafficking of transcription factors; 4. Subnuclear localization and intranuclear trafficking of transcription factors; Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit; Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein; 5. Analysis of ligand dependent nuclear accumulation of Smads in TGF-b; signaling; Douglas A. Chapnick and Xuedong Liu; 6. Raf/MEK/MAPK signaling stimulates the nuclear translocation and trans-; activating activity of FOXM1; Richard Y.M. Ma, Tommy H.K. Tong, Wai Ying Leung; and Kwok-Ming Yao; 7. Coupling of dephosphorylation and nuclear export of Smads in TGF-b; signaling; Fangyan Dai, Xueyan Duan, Yao-Yun Liang, Xia Lin; and Xin-Hua Feng; 8. Assessing sequence-specific DNA binding and transcriptional activity of; STAT1 transcription factor; Thomas Meyer and Uwe Vinkemeier; 9. Analysis of nuclear export using photoactivatable GFP fusion proteins and; interspecies heterokaryons; Kerry-Ann Nakrieko, Iordanka A. Ivanova and Lina Dagnino; 10. Determination of nuclear localization signal sequences for Kruppel-like; factor 8; Tina S. Mehta, Farah Monzur and Jihe Zhao; 11. Methods to measure nuclear export of beta-catenin using fixed and live cell; assays; Manisha Sharma and Beric R. Henderson; 12. Imaging of transcription factor trafficking in living cells: lessons from; corticosteroid receptor dynamics; Mayumi Nishi; Part III. Post-Translational Modifications and Impact on Function; 13. Hypoxia-inducible factors: posttranslational crosstalk of signalling pathways; Elitsa Y. Dimova and Thomas Kiezmann; 14. The basic helix-loop-helix leucine-zipper gene Mitf: analysis of alternative; promoter choice and splicing; Kapil Bharti, Julien Debbache, Xin Wang and Heinz Arnheiter; 15. Phosphorylation control of nuclear receptors; Sebastien Lalevee, Christine Ferry and Cecile Rochette-Egly; 16. Regulation of Krupple-like factor 5 by targeted protein degradation; Ceshi Chen. 17. Post-translational control of ETS transcription factors: detection of modified; factors at target gene promoters; Li Li, Janice Saxton and Pete E. Shaw; 18. Integration of protein kinases into transcription complexes: identifying; components of immobilized in vitro pre-initiation complexes; Hong-Mei Zhang, Stephanie Vouier, Glenn Hodgson; and Peter E, Shaw; 19. Posttranslational modification of p53 by ubiquitin; Chunhong Yan; 20. Phosphorylation-dependent regulation of SATB1, the higher-order chromatin; organizer and global gene regulator; Dimple Notani, Amita S. Limaye, Pavan Kumar and Sanjeev Galande!; Part IV. Protocols for Optimization of Functional Assays; 21. In vivo and in vitro tools to identify and study transcriptional regulation of; USF-1 target genes; Marie-Dominique Galibert and Sebastien Corre; 22. Measuring the absolute abundance of the Smad transcription factors using; quantitative immunoblotting; David C. Clarke and Xuedong Liu; 23. Flow cytometry analysis of transcription factors in T lymphocytes; Diana I. Albu, Danielle Califano and Dorina Avram; 24. Identification of specific Protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family; Marie-Dominique Galibert and Yorann Baron

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