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In the last few years, significant breakthroughs in transcription research has expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells.
This book describes state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function.
Table of Contents; Preface; Contributors; Part I. Transcription Factor Trafficking and Post-Translational Modifications: Overviews of Mechanisms; 1. A review of post-translational modifications and sub-cellular localization of Ets; transcription factors: possible connection with cancer and; involvement in the hypoxic reponse; Celine Charlot, Helene Dubois-Pot, Tsvetan Serchov, Yves Tourette; And Bohdan Wasylyk; 2. Regulation of transcription factor function by targeted protein degradation: an; overview focusing on p53, c-Myc and c-jun; Jukka Westermarck; 3. Review of molecular mechanisms involved in the activation of the Nrf2-ARE; signaling pathway by chemopreventive agents; Aldo Giudice, Claudio Arra and Maria C. Turco; Part II. Cytoplasmic-nuclear trafficking of transcription factors; 4. Subnuclear localization and intranuclear trafficking of transcription factors; Sayyed K. Zaidi, Ricardo F. Medina, Shirwin M. Pockwinse, Rachit; Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein; 5. Analysis of ligand dependent nuclear accumulation of Smads in TGF-b; signaling; Douglas A. Chapnick and Xuedong Liu; 6. Raf/MEK/MAPK signaling stimulates the nuclear translocation and trans-; activating activity of FOXM1; Richard Y.M. Ma, Tommy H.K. Tong, Wai Ying Leung; and Kwok-Ming Yao; 7. Coupling of dephosphorylation and nuclear export of Smads in TGF-b; signaling; Fangyan Dai, Xueyan Duan, Yao-Yun Liang, Xia Lin; and Xin-Hua Feng; 8. Assessing sequence-specific DNA binding and transcriptional activity of; STAT1 transcription factor; Thomas Meyer and Uwe Vinkemeier; 9. Analysis of nuclear export using photoactivatable GFP fusion proteins and; interspecies heterokaryons; Kerry-Ann Nakrieko, Iordanka A. Ivanova and Lina Dagnino; 10. Determination of nuclear localization signal sequences for Kruppel-like; factor 8; Tina S. Mehta, Farah Monzur and Jihe Zhao; 11. Methods to measure nuclear export of beta-catenin using fixed and live cell; assays; Manisha Sharma and Beric R. Henderson; 12. Imaging of transcription factor trafficking in living cells: lessons from; corticosteroid receptor dynamics; Mayumi Nishi; Part III. Post-Translational Modifications and Impact on Function; 13. Hypoxia-inducible factors: posttranslational crosstalk of signalling pathways; Elitsa Y. Dimova and Thomas Kiezmann; 14. The basic helix-loop-helix leucine-zipper gene Mitf: analysis of alternative; promoter choice and splicing; Kapil Bharti, Julien Debbache, Xin Wang and Heinz Arnheiter; 15. Phosphorylation control of nuclear receptors; Sebastien Lalevee, Christine Ferry and Cecile Rochette-Egly; 16. Regulation of Krupple-like factor 5 by targeted protein degradation; Ceshi Chen. 17. Post-translational control of ETS transcription factors: detection of modified; factors at target gene promoters; Li Li, Janice Saxton and Pete E. Shaw; 18. Integration of protein kinases into transcription complexes: identifying; components of immobilized in vitro pre-initiation complexes; Hong-Mei Zhang, Stephanie Vouier, Glenn Hodgson; and Peter E, Shaw; 19. Posttranslational modification of p53 by ubiquitin; Chunhong Yan; 20. Phosphorylation-dependent regulation of SATB1, the higher-order chromatin; organizer and global gene regulator; Dimple Notani, Amita S. Limaye, Pavan Kumar and Sanjeev Galande!; Part IV. Protocols for Optimization of Functional Assays; 21. In vivo and in vitro tools to identify and study transcriptional regulation of; USF-1 target genes; Marie-Dominique Galibert and Sebastien Corre; 22. Measuring the absolute abundance of the Smad transcription factors using; quantitative immunoblotting; David C. Clarke and Xuedong Liu; 23. Flow cytometry analysis of transcription factors in T lymphocytes; Diana I. Albu, Danielle Califano and Dorina Avram; 24. Identification of specific Protein/E-box-containing DNA complexes: lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family; Marie-Dominique Galibert and Yorann Baron