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Academic & Professional Books  Organismal to Molecular Biology  Genetics

Transgenic Techniques

Out of Print
Edited By: Alan Clarke
Publisher: Wiley-Blackwell
Transgenic Techniques
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  • Transgenic Techniques ISBN: 9780896036963 Hardback Dec 2000 Out of Print #111308
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About this book

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.


Biology and Regulation of the Matrix Metalloproteinases, Substrate Specificity of Matrix Metalloproteinases, The Tissue Inhibitors of Metalloproteinases (TIMPs): Biology and Regulation, Matrix Metalloproteinases in Cancer, Hydroxamic Acid Matrix Metalloproteinase Inhibitors, Pinomastat, A Potent and Selective Matrix Metalloproteinase Inhibitor: Preclinical and Clinical Development for Oncology, A Potent Non-Peptidic Matrix Metalloproteinase Inhibitor: Discovery of BAY 12-9566, D1927 and D2163: Novel Mercaptoamide Inhibitors of Matrix Metalloproteinases, Matrix Metalloproteinase Inhibitor Research at Boehringer Mannheim, Therapeutic Applications of Matrix Metalloproteinase Inhibitors Outside of Oncology,

Customer Reviews

Out of Print
Edited By: Alan Clarke
Publisher: Wiley-Blackwell
Media reviews
From Reviews of the First Edition a must in any laboratory - Cellular and Molecular Biology should be useful to anyone currently involved in using transgenesis as a technique - Journal of Heredity Second Edition: ...the book remains an impressive and interesting book that will be a useful reference to molecular and developmental geneticists at all levels and potential tool for teaching graduate students and advanced undergraduates. - Human Genetics It will provide a valuable source of information. It will also remind you of the enormous potential that transgenesis has to offer the biologist. Transgenic research will underpin many future applications from evaluating viral vectors for gene therapy and vaccination to bioreactors for the production of proteins, from models of disease to screening tools for biological activity, from genome mapping to gene function. This book details protocols to let you participate in this. Cloning and Stem Cells
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