Established almost 30 years ago, "Methods in Microbiology" is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, "Methods in Microbiology" will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research. This title focuses on the methods most useful for the functional analysis of yeast genes. It includes advice on different experimental approaches. It covers the bioinformatics of the now fully sequenced Saccharomyces cerevisiae genome. It covers approaches useful for analysis of genes in Saccharomyces cerevisiae, as well as other yeast species. It includes techniques for mutagenesis, transcript analysis, transposon tagging and use of reporter genes. It describes the use of tools for studying post-translational modifications in yeast. It has essential informed coverage of yeast mutant and plasmid collections. It includes useful appendices with handy basic yeast recipes and www addresses. It discusses phenotypic analysis of yeast mutants at cell and biochemical levels.
Praise for the Volume "The book is far more than a cookbook: it seeks to place techniques and experimental strategies in some critical perspective, and expert investigators provide an overview of the state of the art in many areas... it is a compendium packed with information, insights, and discussions of potential pitfalls and limitations, all provided by experienced practitioners. The book successfully brings together a diffuse literature on sets of tools, technologies, and know-how in an accessible format and represents a sound investment for anyone interested in gene analysis." --YEAST "This is another excellent addition to the Methods in Microbiology Series... This is an outstanding resource for both investigators and graduate students working in the field of yeast gene function." --DOODY'S PUBLISHING REVIEWS Praise for the Series "An invaluable resource for the detailed analysis of cellular gene function and genome architecture." --NATURE
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