About this book
Established almost 30 years ago, "Methods in Microbiology" is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, "Methods in Microbiology" will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research. This title focuses on the methods most useful for the functional analysis of yeast genes. It includes advice on different experimental approaches. It covers the bioinformatics of the now fully sequenced Saccharomyces cerevisiae genome. It covers approaches useful for analysis of genes in Saccharomyces cerevisiae, as well as other yeast species. It includes techniques for mutagenesis, transcript analysis, transposon tagging and use of reporter genes. It describes the use of tools for studying post-translational modifications in yeast. It has essential informed coverage of yeast mutant and plasmid collections. It includes useful appendices with handy basic yeast recipes and www addresses. It discusses phenotypic analysis of yeast mutants at cell and biochemical levels.
S.G. Oliver, Introduction to Functional Analysis of the Yeast Genome. E.J. Louis, Whole Chromosome Analysis. H.W. Mewes, D. Frishman, A. Zollner, and K. Heumann, The Bioinformatics of the Yeast Genome. R.D. Gietz and R.A. Woods, Transformation of Yeast by the Lithium Acetate/Single-Stranded Carrier DNA/PEG Method. A. Wach, A. Brachat, C. Rebischung, S. Steiner, K. Pokorni, S. te Heesen, and P. Philippsen, PCR-based Gene Targeting in Saccharomyces cerevisiae. M.J.R. Stark, Studying Essential Genes--Generating and Using Promoter Fusions and Conditional Alleles. D.J. Garfinkel, M.J. Curcio, and V. Smith, Ty Mutagenesis. A.J.P. Brown, L.M. Furness, and D. Bailey, Transcript Analysis. N. Koloteva, J.M.X. Hughes, and J.E.G. McCarthy, Reporter Genes and Their Use in Studying Yeast Gene Expression. P. Ross-Macdonald, A. Sheehan, C. Friddle, G.S. Roeder, and M. Snyder, Transposon Tagging I: A Novel System for Monitoring Protein Production, Function and Localization. M. Bolotin-Fukuhara, Transposon Tagging II: Exploration of Gene Function and Regulatory Networks in Yeast with the Mini-Mu Transposon. I.M. Hagan and C.J. Stirling, Immunological Approaches to the Study of Protein Localisation in Yeast. F.M. Klis, A.F.J. Ram, R.C. Montijn, J.C. Kapteyn, L.H.P. Caro, J.H. Vossen, M.A.A. Van Berkel, S.S.C. Brekelmans, and H. Van den Ende, Posttranslational Modifications of Secretory Proteins. M.J.R. Stark, Studying Post-Translational Modifications in Yeast-Intracellular Proteins. J. Rosamond, Two-Hybrid Analysis of Protein-Protein Interactions in Yeast. J. de la Cruz, M.C. Daugeron, and P. Linder, 'Smart' Genetic Screens. B. Teusink, F. Baganz, H.V. Westerhoff, and S.G. Oliver, Metabolic Control Analysis as a Tool in the Elucidation of the Function of Novel Genes. W.H. Mager, K. Maurer, and P.W. Piper, Identifying Stress Genes. M.F. Tuite, I. Stansfield, and R.J. Planta, Identifying Genes Encoding Components of the Protein Synthesis Machinery of the Yeast Saccharomyces cerevisiae. M. Molina, H. Martin, M. Sanchez, and C. Nombela, MAP Kinase-Mediated Signal Transduction Pathways. P.T. Magee, The Analysis of the Candida albicans Genome. P.A. Moore, Applications of Yeast in the Discovery of Human Gene Function. K.D. Entian and P. Kotter, Yeast Mutant and Plasmid Collections. A. Brown and M.F. Tuite, Yeast Gene Analysis in the 21st Century. Appendices: R.J. Planta, I. Stansfield, and M.F. Tuite, The Ribosomal Protein Genes of Saccharomyces cerevisiae. A.J.P. Brown and M.F. Tuite, Yeast Growth. A.J.P. Brown, Useful World Wide Web Addresses for Yeast Researchers. M.F. Tuite, Nomenclature for Yeast Genes and Proteins. Subject Index.
Alistair Brown has led an active research group in yeast molecular biology for over 15 years. Having started at Glasgow University, his group is now based at Aberdeen University, studying gene function and regulation in Saccharomyces cerevisiae and the control of morphogenesis in Candida albicans. The Aberdeen Candida Group is now one of the largest academic groups studying the molecular physiology of C. albicans. Mick Tuite is a Professor of Molecular Biology in the Department of Biosciences at the University of Kent, UK He has published over 100 research papers and reviews in the area of yeast molecular biology and genetics and is a member of the editorial board of the journals Yeast and Molecular Microbiology.