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Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.
Contents
Preface Contributors THE PROTOPLAST SYSTEM The Fungal Cell Wall Vladimir FarkaA! Mycolytic Enzymes John F. Peberdy Factors Influencing Protoplast Isolation Barry Davis Protoplasts from Fungal Spores Cees J. Bos Protoplasts from Entomophthorales Richard A. Nolan PROTOPLASTS AS TOOLS IN PHYSIOLOGICAL AND BIOCHEMICAL STUDIES Cell Wall Regeneration and Protoplast Reversion Oldrich Necas and Augustin Svoboda The Protoplast Membrane and Antifungal Drugs David Kerridge Metabolic Properties of Protoplasts Susan Isaac Protoplasts and Secondary Metabolism Joan W. Bennett PROTOPLASTS AS GENETIC TOOLS Protoplast Fusion and Genetic Analysis in Cephalosporium John A. Birkett and Paul F. Hamlyn Protoplast Fusion and Incompatibility in Aspergillus James H. Croft Interspecies Hybridization after Protoplast Fusion in Aspergillus Ferenc Kevei and John F. Peberdy Protoplast Fusion and Interspecies Hybrization in Penicillium Jozef Anne and John F. Peberdy Protoplast Fusion in Yeasts Lajos Ferenczy Transfer of Cytoplasmic Genetic Elements by Protoplast Fusion Lajos Ferenczy Some Aspects of Yeast Transformation Albert Hinnen Perspectives for Protoplasts in Genetics of Industrially Important Fungi Christopher Ball Index
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