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Academic & Professional Books  Botany  Plants & Botany: Biology & Ecology

Agrobacterium From Biology to Biotechnology

Edited By: Tzvi Tzfira and Vitaly Citovsky
750 pages, Figs, tabs
Publisher: Springer-Verlag
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  • Agrobacterium ISBN: 9780387722894 Hardback Feb 2008 Usually dispatched within 1-2 weeks
Selected version: £219.99
About this book Contents Customer reviews Biography Related titles

About this book

Agrobacterium is a plant pathogen which causes the "crown-gall" disease, a neoplastic growth that results from the transfer of a well-defined DNA segment ("transferred DNA", or "T-DNA") from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades.

Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.


Agrobacterium: a disease-causing bacterium.- A brief history of research on Agrobacterium tumefaciens: 1900-1980.- Agrobacterium and plant biotechnology.- The Agrobacterium tumefaciens C58 genome.- Agrobacterium?taxonomy of plant-pathogenic Rhizobium species.- The initial steps in Agrobacterium tumefaciens pathogenesis: chemical biology of host recognition.- Agrobacterium-host attachment and biofilm formation.- Production of a mobile T-DNA by Agrobacterium tumefaciens.- Translocation of oncogenic T-DNA and effector proteins to plant cells.- Intracellular transport of Agrobacterium T-DNA.- Mechanisms of T-DNA integration.- Agrobacterium tumefaciens-mediated transformation: patterns of T-DNA integration into the host genome.- Function of host proteins in the Agrobacterium-mediated plant transformation process.- The oncogenes of Agrobacterium tumefaciens and Agrobacterium rhizogenes.- Biology of crown gall tumors.- The cell-cell communication system of Agrobacterium tumefaciens.- Horizontal gene transfer.- Agrobacterium-mediated transformation of non-plant organisms.- The bioethics and biosafety of gene transfer.- Agrobacterium-mediated gene transfer: a lawyer?s perspective.

Customer Reviews


Dr. Tzvi Tzfira is an Assistant Professor in the Department of Molecular, Cellular, and Developmental Biology at the University of Michigan. Dr. Vitaly Citovsky is a Professor in the Department of Biochemistry and Cell Biology at Stony Brook University.

Edited By: Tzvi Tzfira and Vitaly Citovsky
750 pages, Figs, tabs
Publisher: Springer-Verlag
Media reviews

"A compilation of many aspects of Agrobacterium research, with each chapter authored by experts in the field. Though the book is heavily oriented toward the genetic/molecular basis of the gene transfer process, it includes the history of Agrobacterium research, nomenclature, and basic biology of the bacterial/plant interaction. The final chapters review bioethics and legal aspects of Agrobacterium-mediated gene transfer. Summing Up: Recommended. Graduate students and researchers/faculty."
– L. M. Baird, Choice, Vol. 45 (11), August, 2008

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