Books  Ecological & Biological Sciences  Animal & General Biology  Biochemistry & Molecular Biology 

Animal Cell Culture and Technology

Handbook / Manual

By: M Butler


Paperback | Dec 2003 | Edition: 2 | #150745 | ISBN: 1859960499
Availability: Usually dispatched within 6 days Details
NHBS Price: £32.00 $42/€36 approx

About this book

The Basics series provide the ideal introduction to new techniques in the laboratory. This series of books will allows you to use the techniques described, and to manipulate them to suit your own basic requirements. Some basic theory is given, but is linked to simple explanations and suggestions for which protocols may be suitable for you to use. Each book answers the important questions of 'why' and 'how' experiments are carried out. Particular attention is given to the interpretation of data and the possible sources of errors and artifacts that may be encountered. If you are a students new to the technique involved, or even if you are an experienced worker in the field, this series of use-friendly guides to successful laboratory techniques will be ideal for you. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for scientists intending to initiate some work in this field. New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock (teleomeres) of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use.


1. The Development of Animal Cell Technology 2. Characteristics of Cells in Culture Protocols For Deriving Primary Cell Culture. Selecting and Cloning Cells 3. Growth and Maintenance of Cells in Culture Protocols For Sub-Culturing a Cell Line. Establishing a Stationary Culture. Establishing a Stirred Microcarrier Culture. Adapting Cells to Serum-Free Formulations 4. Cell Line and Culture Monitoring Protocols for Cell Counting. Karyotyping. Isozyme Analysis 5. Genetic Engineering of Animal Cells Protocols for Transfecting a Cell Line. Expressing a Protein 6. Hybridoma Technology Protocols for Cell Fusion. Producing Monoclonal Antibodies 7. Glycosylation of Proteins Protocols for Analysing Glycans Attached to Proteins 8. Bioreactors For Cell Culture Protocols for Scale-Up in a Stirred-Tank Bioreactor 9. Modes of Cell Culture Protocol for Establishing a Continuous Culture 10. Production of Biologicals from Cell Cultures.

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