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This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
Animal Cell Culture - Essential Methods John Davis (Editor) 1. The Cell Culture Laboratory Susan Clarke and Janette Dillon 2. Sterilization Peter Roberts 3. The Microscopy of Living Cells Colin Gray and Daniel Zicha 4. Basic Techniques and Media, the Maintenance of Cell Lines, and Safety John Davis 5. Serum-Free Culture Media David Jayme and Stephen Gorfien 6. Cryopreservation and Cell Banking Glyn N. Stacey, J. Ross Hawkins and Roland A. Fleck 7. Primary Culture of Specific Cell Types, and the Establishment of Cell Lines Kee Woei Ng, Jan-Thorsten Schantz, and Chris Morris 8. Cloning John Clarke, Alison Porter and John Davis 9. The Quality Control of Cell Lines and the Prevention, Detection and Cure of Contamination Peter Thraves and Cathy Rowe 10. Systems for Culture Scale-Up Jennifer Halsall and John Davis 11. Good Laboratory Practice (GLP) in the Cell Culture Laboratory Barbara Orton
Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.