Cells: A Laboratory Manual (3-Volume Set)

About this book

Having identified a gene product, how do you determine what it does? This manual seeks to answer this question, with a collection of strategies and techniques for the study of cellular components. It contains step-by-step protocols, ranging widely from basic culture of vertebrate and non-vertebrate cells, through advanced detection methods using immunological, cytochemical and molecular in-situ approaches, and imaging techniques such as confocal and deconvolution microscopy.

Contents

Volume 1 Culture and biochemical analysis of cells: cell culture metabolic labelling of cells/organisms; subcellular fractionation; protein separation and identification; systems for protein expression; antibodies as tools in cell biology. Volume 2 Light microscopy and cell strucure: preparation of live cells for observation by light microscopy; preparation of macromolecules and introduction into cells; light and epifluorescence microscopy; confocal microscopy and deconvolution techniques. Volume 3 Subcellular localization of genes and their products: visualization of proteins, organelles and gene expression; in situ hybridization; electron microscopy; immunoelectron microscopy.

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