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Checklist of Macao Bryophytes documents 103 species of bryophytes occurred in Macao, of which liverworts contain 27 species in 22 genera and 14 families; hornworts 4 species in 3 genera and 2 families; and mosses 72 species in 38 genera and 17 families. Checklist of Macao Bryophytes is published in a bilingual edition (Chinese and English). For each species, information on Chinese name, scientific name, references, collection number, localities, habitats, altitude, and worldwide distributions was provided. It is intended to be a tool for the study of bryophyte diversity of Macao.