Biological Electron Microscopy Theory, Techniques, and Troubleshooting

In this second edition of his 1992 hardcover text and 1993 spiral-bound lab manual on Biological Electron Microscopy, Michael Dykstra has expended considerable effort to merge the two earlier volumes into a more readable and usable single volume and also to update them and add considerable new material. Happily, the result is a first rate, comprehensive book that will be useful for both teaching beginning students and as a reference book for experienced researchers. In all chapters, new materials have been added in the text and referenced at the chapter ends, and where appropriate, useful web sites have been indicated where additional information may be obtained. All of the excellent illustrative photographs from the first edition have been retained. As before, the chapters on microscope construction and operation are very well done and will be invaluable in teaching. Cautionary statements are made throughout about handling and disposal of the hazardous materials used in EM labs. Relevant journals, societies, and equipment suppliers are listed in appendices. In general, this single volume is a welcome addition to the literature available on biological microscopy. Its publication offers support for the idea that microscopy in all of its guises is still a dynamic and valuable tool for all biologists. (Henry C. Aldrich, Professor Emeritus of Microbiology and Cell Science, University of Florida, Gainesville)