DNA Methylation: Approaches, Methods, and Applications

About this book

A guide to understanding the complex regulatory roles of DNA methylation, this volume describes the relation that this biological mechanism has to gene silencing in disease and explores its promising role in treating cancer. DNA Methylation: Approaches, Methods and Applications describes the relation DNA methylation has to gene silencing in disease, and explores its promising role in treating cancer. The author considers research, clinical, and industry perspectives in this growing field, and by synthesizing previously scattered literature, he offers the subject's first organized and cohesive resource and bibliography.The book is a state-of-the-art reference for understanding the role DNA methylation plays in human disease, particularly cancer. Written by leaders in the field, this exceptional compilation of articles outlines the best techniques to use when addressing questions concerning the cytosine methylation status of genomic DNA. It includes concepts, experimental models, and clinical uses of demethylating agents. The book provides a balance between articles clarifying methodological details and more general review chapters that offer broad biological perspectives on DNA methylation.Covering fundamental theory, technologies, and applications, DNA Methylation: Approaches Methods, and Applications presents the most current research on using DNA methylation to decipher the pathways involved in human disease. This is an invaluable handbook for researchers and clinicians interested in genetics and molecular biology, particularly epigenetic therapies and gene silencing.

Contents

Impact of DNA Methylation in Human Health and Disease; Manel EstellerUses of DNA Methylation in Cancer Diagnosis and Risk Assessment; Carmen Jeronimo, Rui Henrique, David SidranskyDNA Methylation in Imprinting, X-Chromosome Inactivation and Associated Diseases; Maria Rosaria Matarazzo, Maria Strazzullo, Maurizio D'EspositoStudying Mammalian DNA Methylation: Bisulfite Modification; Susan J. ClarkBisulfite PCR-Based Techniques for the Study of DNA Methylation; James G. HermanMicroarray Analysis of DNA Methylation Targets Identified by Methyl-CpG Binding Proteins; Masahiko Shiraishi, Michael W.-Y. Chan, Tim H.-M. HuangMethylation Arbitrarily-Primed PCR and Derived Methodologies; Jordi Frigola, Maria F. Paz, Manel Esteller Miguel Angel PeinadoDiscovering DNA Methylation Differences with Restriction Landmark Genome Scanning; Christoph Plass, Laura J. Rush, Dominic J. Simraglia, Joseph CostelloQuantitative Determination of 5-methylcytosine DNA Content: HPCE and HPLC; Mario F. Fraga, Manel EstellerQualitative Determination of 5-methylcitosine DNA and other Components of the DNA Methylation Machinery: Immunofluorescence and Chromatin Immunoprecipitation; Jesus Espada, Esteban Ballestar and Manel EstellerMouse Models for the Study of DNA Methylation; Nicole M. Sodir, Peter W. LairdDNA Demethylating Agents: Concepts; Jonathan C. Cheng, Daniel J. Weisenberger, Peter A. JonesDNA Demethylating Agents: Preclinical Evaluation as Anticancer Agents; Robert BrownDNA Demethylating Agents: Clinical Uses; Michael Lnbbert, Jens Hasskarl, Rainer Claus

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