Neurons: Methods and Applications for the Cell Biologist

About this book

This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. It contains culture methodology for more than a dozen types of CNS and PNS neurons. It includes most recent and reliable techniques from expert practitioners for specific experimental applications. It addresses the latest strategies for transfecting neurons.

Contents

Comparing the Properties of Neuronal Culture Systems Growing and Working with Peripheral Neurons Dissection and Culturing of Chick Ciliary Ganglion Neurons The Culture of Chick Forebrain Neurons Growing and Working with Spinal Motor Neurons Avian Purkinje Neuronal Cultures Culturing Hippocampal and Cortical Neurons Working with Xenopus Spinal Neurons in Live Cell Cultures Culturing Neurons from the Snail Helisoma The Ti1 Pioneer Pathway Techniques to Dissect Cellular and Subcellular Function in Drosphila Nervous System PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.

Customer Reviews

Biography

Edited by Peter J. Hollenbeck and Dr. James R. Bamburg