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Academic & Professional Books  Organismal to Molecular Biology  Genetics

DNA Cloning: A Practical Approach, Volume 2 Expression Systems

Handbook / Manual
Edited By: BD Hames and D Glover
253 pages, 20 b/w photos, 38 illus
Publisher: IRL Press
DNA Cloning: A Practical Approach, Volume 2
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  • DNA Cloning: A Practical Approach, Volume 2 ISBN: 9780199634781 Edition: 2 Paperback May 1995 Usually dispatched within 2-3 weeks
    £70.00
    #38566
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About this book Contents Customer reviews Related titles

About this book

Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories.

Contents

1. Screening expression libraries with antibody and protein probes; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies; 3. Purification of over-produced proteins from E.coli cells; 4. Production of monoclonal antibodies against proteins expressed in E.coli; 5. Expression of cloned genes in yeast; 6. Intraction trap cloning with yeast; 7. The baculovirus expression system

Customer Reviews

Handbook / Manual
Edited By: BD Hames and D Glover
253 pages, 20 b/w photos, 38 illus
Publisher: IRL Press
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