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This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision.