Fish Cytogenetic Techniques Ray-Fin Fishes and Chondrichthyans

About this book

Recent advances in fish cytogenetics have enhanced the interest in chromosome analysis in both fundamental (systematics and comparative genomics among fishes and other vertebrate groups) and applied (aquaculture, conservation and response to pollutants, whole genome sequencing of model fish species) research. Although the genomic material, the chromosomes, is basically the same in the various organisms, experience has clearly shown that fish chromosomes have to be handled with specific protocols.

In laboratories around the world, traditional cytogenetic techniques and molecular cytogenetics have seen a vast improvement but the technical details and protocols are often not published in scientific journals because of constraints in format and size and therefore they are not accessible to the whole scientific community. These shortcomings are very well known to fish cytogeneticists and the idea to prepare a specific manual was first discussed during the first "Workshop of Fish Cytogenetics" held in Concarneau (France), in 1992. Resulting from the above workshop, a draft compilation of the techniques and protocols in fish cytogenetics available at that time had been produced and distributed privately, but never published. There does not exist, at present, any published handbook or manual specifically dealing with fish cytogenetics.

Fish Cytogenetic Techniques fills this void by bringing together and updating the main techniques so that the traditional methods can be standardized. Moreover, Fish Cytogenetic Techniques includes novel developments or improvements; some of the protocols provided here are practically unknown to the scientific community and even now unpublished.

Contents

Teleost Fish Handling and Transport under Reduced Stress Conditions; Benoit Aupérin and Jean-François Baroiller

Storage of Karyotyped Voucher Specimens and their Molecular Identification; A. Dettai and P. Pruvost

Direct Chromosome Preparation from Freshwater Teleost Fishes; L.A.C. Bertollo, M.B. Cioffi and O. Moreira-Filho

Direct Mitotic Chromosome Preparations from Chondrichthyan Tissues; Lucia Rocco

Mitotic Chromosome Preparations of Freshwater Stingrays; Vanessa Paes da Cruz and Fausto Foresti

Direct Chromosome Preparation from Regenerating Fish Fin Tissue; M. Völker and P. Ràb

Direct Chromosome Preparations from Embryos and Larvae; M. Völker and P. Ràb

Establishment of Sturgeon Primary Cell Lines; Francesco Fontana

Teleost Fish Lymphocyte Culture; S. Salvadori, E. Coluccia and A.M. Deiana

Rapid Fibroblast Culture for Teleost Fish Karyotyping; M. Rábová, R. Monteiro, M.J. Collares-Pereira and P. Ráb

Cephalic Kidney and Spleen Cell Culture in Antarctic Teleosts; Olivier Rey, Angélique d’Hont, Jean-Pierre Coutanceau, Eva Pisano, Stefan Chilmonczyk and Catherine Ozouf-Costaz

Meiotic Surface-spread and Immunodetection for Synaptonemal Complex Proteins in Teleost fish; C. Araya-Jaime , E. Alves-Serrano, C. Oliveira and F. Foresti

C-banding; S. Salvadori, E. Coluccia and A.M. Deiana

Sequential Chromosome Banding in Fishes; M. Rábová, M. Völker, Š. Pelikánová and P. Ráb

Standard Fluorescence in Situ Hybridization Procedures; Céline Bonillo, Jean-Pierre Coutanceau, Helena D’Cotta, Laura Ghigliotti, Catherine Ozouf-Costaz and Eva Pisano

Characterization of Fish Genomes by GISH and CGH; R. Symonová, A. Sember, Z. Majtánová and P. Ráb

Fish Chromosome Microdissection and its Utilization in the Synthesis of Fluorescent in situ Hybridization Probes; A. Laudicina and M.C. Mühlmann

Microdissection and Chromosome Painting; M. R. Vicari, J. C. Pansonato-Alves and F. Foresti

Extending DNA Fibers and Fiber-FISH; C. Ozouf-Costaz, J.P. Coutanceau, A. D’Hont, D. Chassoux and G. Lecointre

In vivo Micronucleus Test for Detecting Response of Teleost Fish to Environmental Genotoxic Exposure; Phan van Ngan, Vicente Gomes, Matia José A.C.R. Passos and Arthur José da Silva Rocha

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