By: C Michels
240 pages, Figs, tabs
This advanced textbook uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis.
...Students new to yeast genetics and those of us keeping abreast of fusing disciplines should add this publication to our reading list... (The Biochemist, 4 October 2002) " a useful addition to the literature " (Biologist, Vol.50. No.2, 2003) "...a valuable addition to the reference library of any research laboratory at universities, research institutes and the industry." (Acta Biotechnologica, Vol.23, No.2 3, 2003) "...The aim of every teacher is to inspire students to move down from the stands, step up to the plate, and start hitting on their own. This book is a wonderful tool to help in that quest." (Cell Biology Education, 16 June 2003)
Introduction.SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM. Saccharomyces cerevisiae as a Genetic Model Organism Techniques in Cell and Molecular Biology Saccharomyces Cell Structure. SECTION II: TECHNIQUES OF GENETIC ANALYSIS. Mutant Hunts--To Select or to Screen (Perhaps Even by Brute Force). Complementation Analysis: How Many Genes are Involved? Epistasis Analysis. Gene Isolation and Analysis of Multiple Mutant Alleles. Suppression Analysis. Enhancement and Synthetic Phenotypes Two Hybrid Analysis Advanced Concepts in Molecular Genetic Analysis Genomic Analysis SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE. Case Study I: Glucose Sensing and Signaling Mechanisms in Saccharomyces. Case Study II: Secretion, Exocytosis, and Vesicle Trafficking in Saccharomyces. Case Study III: The Cell Division Cycle of Saccharomyces. Case Study IV: Mating type Pheromone Response Pathway of Saccharomyces. Index.
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