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Third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.
Foundations of Confocal Scanned Imaging in Light Microscopy.- Fundamental Limits in Confocal Microscopy.- Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM) The Pixilated Image.- Laser Sources for Confocal Microscopy.- Nonlaser Light Sources: Not Using Laser Light for Confocal Microscopy and How to Use Laser Light When It's All You Have.- Objective Lenses for Confocal Microscopy.- The Specimen Illumination Path and Its Effect on Image Quality.- The Intermediate Optical System of Laserscanning Confocal Microscopes.- Intermediate Optics in Nipkow Disk Microscopes.- The Role of the Pinhole in Confocal Imaging System Photon Detectors for Confocal Microscopy.- The Collection, Processing, and Display of Digital Threedimensional Images of Biological Specimens.- Visualization Systems for Multidimensional CLSM Images.- Mapping and Measuring Surfaces Using Relection Confocal Microscopy.- 22 additional articles.-Index.