Histochemical and Cytochemical Methods of Visualization

About this book

Histochemical and Cytochemical Methods of Visualization provides the essential techniques used for histochemical investigations in both light and transmission electron microscopy. The text presents both classic techniques as well as specialized methods.

It first presents theoretical material in order to provide a conceptual framework. It then covers material, reagents, solutions, preparation, and storage. The author also provides a glossary and an index for further reference.

Contents

HISTOLOGICAL AND HISTOCHEMICAL METHODS IN LIGHT MICROSCOPY
Classical Methods of Visualization, Jean-Marie Exbrayat
General Principles of Histology
Preparation of Tissues (Preservation, Conservation, Embedding, Sectioning)
Methods of Staining
Mounting and Observation
Histochemical Methods, Jean-Marie Exbrayat
General Histochemical Methods of Staining
Histochemistry of Carbohydrates
Histochemistry of Nucleic Acids
Histochemistry of Protids
Histochemistry of Lipids
Histochemistry of Nucleic Acids
Biogenic Amines Staining
Mineral Detection
Histoenzymatic Methods, Jeanne Estabel
CYTOCHEMICAL METHODS IN ELECTRON MICROSCOPY
General Techniques in Electron Microscopy, Jeanne Estabel
Preparation of tissues (preservation, conservation, embedding,
sectioning)
Methods of staining
Mounting and observation
General Cytochemical Techniques
Cytochemical method (carbohydrates, lipids, ... ), Jean-Marie Exbrayat
Acetylation Method, Marc Thiry
Ultrastructural Cytoenzymology, Chantal De Chastellier
PREPARATION OF PRODUCTS
EXAMPLES OF STAINING METHODS
Glossary
Index

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Biography

Jean-Marie Exbrayat is a Professor at the Catholic University of Lyon and EPHE Lyon, France.