Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish

About this book

28 plates with colour photos and colour illustrations

Clarifying much of the inconsistency currently encountered in the scientific literature, Histological Analysis of Endocrine Disruptive Effects on Small Laboratory Fish helps to standardize the interpretation of results from aquatic bioassays and field observations by describing basic biological function and contrasting normal histology with histopathological conditions in a variety of fish species. The authors represent the leaders of small fish gonadal histopathology, and they incorporate views and ideas from other experts.

Contents

Preface   xi
Acknowledgments   xiii
Contributing Authors   xv

1 Introduction   1
2 Fish Species of Interest   7
3 Sexual Determination, Differentiation, and Gonadal Development   19
4 Female Gonad Anatomy and Morphology   66
5 Male Gonad Anatomy and Morphology   88
6 Endocrine-Disrupting Compounds   115
7 Determination of Effects of Exogenous Hormones and Endocrine-like Active Compounds   247
8 Evaluation of Effects in Fish Gonads   254
9 Experimental Design and Statistics   270
10 Conclusions   295

Appendix: Fish Preparation and Microdissection of Organs   301
A.1 Fish Preparation   301
A.2 Microdissection of Organs   302
A.3 Tissue Fixation   303
A.4 Embedding   307
A.5 Tissue Sectioning   310
A.6 Sample Mounting   311
A.7 Tissue Slide Staining   312
A.8 Final Processing   315
A.9 Examples   316
A.10 Summary   317

References   318
Index   319

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Biography

Daniel R. Dietrich, PhD, is a Professor and Head of the Human and Environmental Toxicology Group, Faculty of Sciences of the University of Konstanz, Konstanz, Germany. Heiko O. Krieger, MSc, is a scientific research assistant and PhD student in the Human and Environmental Toxicology Group, Faculty of Sciences of the University of Konstanz, Konstanz, Germany.