435 pages, 128 illus
`Imaging cellular and molecular biological function' provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions.
From the reviews: "Imaging Cellular and Molecular Biological Functions presents essays by experts in the field for scientists and students who are interested in all aspects of imaging, including development and applications." (Spencer L. Shorte and Friedrich Frischknecht, Biophotonics International, April, 2008) "This 450-page, color-illustrated book summarizes the latest technology. It is clearly structured in three sections. ! We can recommend this book to young researchers and imaging specialists ! thanks not only to the numerous illustrations that help clarify the text, but also to the extensive collection of sources relating to each chapter." (Microscopy and Imaging, November, 2008)
I Considerations for Routine Imaging.- 1 Entering the Portal: Understanding the Digital Image Recorded Through a Microscope.- 2 Quantitative Biological Image Analysis.- 3 The Open Microscopy Environment: a Collaborative Data Modeling and Software Development Project for Biological Image Informatics.- 4 Design and Function of a Light-Microscopy Facility.- II Advanced Methods and Concepts.- 5 Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls.- 6 Quantitative FRET Microscopy of Live Cells.- 7 Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells.- 8 Single Fluorescent Molecule Tracking in Live Cells.- 9 From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins. III Cutting-Edge Applications and Utilities.- 10 Towards Imaging the Dynamics of Protein Signalling.- 11 New Technologies for Imaging and Analysis of Individual Microbial Cells.- 12 Imaging Parasites in Vivo.- 13 Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells.- 14 High-Throughput/High-Content Automated Image Acquisition and Analysis.- 15 Cognition Network Technology -- a Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents.- 16 High-Content Phenotypic Cell-Based Assays.
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