Introduction to Plant Biotechnology

About this book

This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation.

For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.

Contents

Part I: Plant Tissue Culture: Introduction; Laboratory Organization; Nutrition Medium; Sterilization Techniques; Types of Culture; Micropropagation; Cell Suspension and Secondary Metabolites; In Vitro Production of Haploids; Protoplast Isolation and Fusion; Somaclonal Variation; Germplasm Storage and Cryopreservationi? Part II: Genetic Material and its Organization: Genetic Material; Organization of DNA and Gene Expressioni? Part III: Recombinant DNA Technology: Basic Techniques; Gene Cloning: Cutting and Joining DNA Molecules; Gene Cloning: Vectors; Gene Cloning: cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences; Polymerase Chain Reaction; In Vitro Mutagenesis; Transposon Genetic Elements and Gene Tagging; Gene Isolation; Molecular Markers and Marker-Assisted Selection; Gene Transfer Methods; Chloroplast and Mitochondrion DNA Transformation; Transgenics in Crop Improvement; Impact of Recombinant DNA Technology; Biosafety Concerns and Regulatory Framework; Genomics; Bioinformatics; Intellectual Property Rights; Appendix; Glossary

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