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The purpose of Light and Video Microscopy is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. It brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope. It clearly develops all ideas from historical and logical foundations.
Laboratory exercises included to assist the reader with practical applications. Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope.
Chapter 1: The Relation Between the Object and the Image
Chapter 2: Geometrical Optics
Chapter 3: Physical Optics
Chapter 4: Bright Field Microscopy
Chapter 5: Phase Contrast and Dark Field Microscopy, Rheinberg and Oblique Illumination
Chapter 6: Photomicrography
Chapter 7: Fluorescence Microscopy
Chapter 8: Polarization Microscopy
Chapter 9: Interference Microscopy
Chapter 10: Differential Interference Microscopy
Chapter 11: Modulation Contrast Microscopy
Chapter 12: Video and Digital Microscopy
Chapter 13: Image Processing and Analysis
Chapter 14: Various Kinds of Microscopes and Accessories
"As such, this book would be an excellent undergraduate-level text on optical microscopy for biologists. It is also valuable to anyone using a light microscope."
– Carol Bayles, Cornell University, Ithaca, NY, USA