Manual of Techniques in Invertebrate Pathology

About this book

The second edition of Manual of Techniques in Invertebrate Pathology is written by an international group of experts that contribute a broad array of techniques for the identification, isolation, culture, bioassay, propagation, and storage of the major groups of entomopathogens. Manual of Techniques in Invertebrate Pathology provides general and specific background to experienced insect pathologists, biologists, and entomologists who work with pathogen groups that are new to them. It is also useful as a laboratory manual for courses in insect pathology and biological control and related areas of study. Safety testing of entomopathogens in mammals and complementary techniques for the preparation of entomopathogens are included as well as broader methods for the study of specimens such as microscopy and molecular techniques.

Manual of Techniques in Invertebrate Pathology concentrates primarily on practical step-by-step aspects of the techniques, but also provides the reader with a short history, rationale for usage, guides to supplemental literature, plus recipes for media, fixatives, and stains. Manual of Techniques in Invertebrate Pathology provides step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens. New edition is fully updated to address changes in the taxonomy of the vast majority of taxa. Manual of Techniques in Invertebrate Pathology includes a discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques.

Contents

1. Initial Handling and Diagnosis of Diseased Invertebrates
2. Basic Techniques in Insect Virology
3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli.
4. Bioassay of Bacterial Entomopathogens Against Insect Larvae
5. Bacteria for use Against Soil-Inhabitating Insects
6. Identification of Entomopathogenic Fungi
7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales
8. Mass Production of Entomopathogenic Hypocreales
9. Methods for the Study of Entomophthorales
10. Preservation of Entomopathogenic Fungal Cultures
11. Research Methods for Entomopathogenic Microsporidia and Other Protists
12. Nematode Parasites, Pathogens and Associates of Insects and Invertebrates of Economic Importance
13. Pathogens and Parasites of Terrestrial Molluscs
14. Testing the Pathogenecity and Infectivity of Entomopathogens to Mammals
15. Complementary Techniques: Preparations of Entomopathogens and Diseased Specimens for More Detailed Study Using Microscopy

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