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Mass Spectrometry of Nucleosides and Nucleic Acids

Edited By: Joseph H Banoub and Patrick A Limbach
504 pages, 255 black & white illustrations, 40 black & white tables
Publisher: CRC Press
Mass Spectrometry of Nucleosides and Nucleic Acids
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  • Mass Spectrometry of Nucleosides and Nucleic Acids ISBN: 9781420044027 Hardback Dec 2009 Usually dispatched within 6 days
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About this book

Nucleic acid research has had a profound impact on molecular biology, resulting in a variety of new disciplines. Mass Spectrometry of Nucleosides and Nucleic Acids puts into perspective the progress in biotechnological research developed over the last few decades. This book provides an overview of the most popular ionization methods, often used in conjunction with tandem mass spectrometry. The text illustrates the diversity of strategies used in the characterization and sequencing of simple nucleosides and nucleotides, covering complex DNA, single strands and duplexes, DNA adducts, RNA oligomers, DNA drug complexes, and posttranscriptional modifications, among other topics.

Contents

Overview of Recent Developments in the Mass Spectrometry of Nucleic Acid and Constituents. Mass Spectrometric Analysis of Deoxyinosines. Tandem Mass Spectrometry of Nucleic Acids. Mass Spectrometric Application in the Study of Cyclic Nucleotides in Biochemical Signal Transduction. Analysis of Urinary Modified Nucleosides by Mass Spectrometry. Mass Spectrometric Determination of DNA Adducts in Human Carcinogenesis. Sequence Distribution of Nucleobase Adducts Studied by Isotope Labeling of DNA-Mass Spectrometry. Electrospray Mass Spectrometry of Noncovalent Complexes between Small Molecule Ligands and Nucleic Acids. Electrospray Ionization-Mass Spectrometry for the Investigation of Protein--Nucleic Acid Interactions. Characterization of Noncovalent Complexes of Nucleic Acids with Peptides and Proteins by Mass Spectrometry. MALDI-TOF Detection of Specific Noncovalent Complexes of Highly Acidic Biomolecules with Pyrenemethylguanidinium. Quantitative Identifi cation of Nucleic Acids via Signature Digestion Products Detected Using Mass Spectrometry. Electrospray Ionization Mass Spectrometry for the Direct Analysis of Modified Nucleosides in Small RNAs. LC-MS/MS for the Examination of the Cytotoxic and Mutagenic Properties of DNA Lesions. Influence of Metal Ions on the Structure and Reactivity of Nucleic Acids. Index.

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Biography

Fisheries and Oceans Canada, Science Branch and Memorial University of Newfoundland, Canada University of Cincinnati, Ohio, USA
Edited By: Joseph H Banoub and Patrick A Limbach
504 pages, 255 black & white illustrations, 40 black & white tables
Publisher: CRC Press
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