Based on collection activities over the past 25 years, a revision of literature, and unpublished materials, an annotated checklist of trees and arborescent species found in and reported from El Salvador, Central America is presented. This is the second of three volumes covering 29 angiosperm plant families (Magnoliaceae to Punicaceae, and Achatocarpaceae) and 3 families of ferns. A total of 289 species and 24 subspecies and varieties are recognised. representing 291 taxa at the most basic rank, of which 205 are native in El Salvador. The checklist includes the correct names, their synonyms as far as they have been in use for Salvadorian taxa, the basionyms, and nomenclatural details for all names cited. Additional data for each taxon include vemacular names, data on local and global distribution, selected information on (potential) utilisation, as Well as detailed literature references from works directly referring to the flora of El Salvador as well as a selection of relevant Central American floras. A total of 1682 herbarium collections are cited in this volume, of which 1426 specimens are located – and have been revised – at LAGU and/or B. The introduction provides a summary of collection activities related to the Jardin Botanico La Laguna as well as an introduction to taxonomic inventories in El Salvador.