RNA: A Laboratory Manual

About this book

So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manual provides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.

Contents

Chapter 1: The Fundamentals
Chapter 2: Purification of RNA from Natural Sources
Chapter 3: Detection and Sequence Determination of Specific RNAs
Chapter 4: Synthesis and Modification of RNA In Vitro
Chapter 5: Uses of Synthetic RNAs
Chapter 6: Testing RNA Substrates for Activity In Vitro
Chapter 7: RNAi-related Techniques
Chapter 8: Genomic Approaches

Appendices
I Reagents
II Stock Solutions
III Nucleases and What They Do
IV Miscellaneous Protocols
V Cautions

Customer Reviews